Streptavidin Conjugation Protocol
CPNs™ can be readily conjugated to proteins using EDC (N-ethyl-N’-dimethylaminopropyl-carbodiimide), which enables linkage of the protein amine groups (-NH2) to the carboxyl groups on the surface of the CPNs™ (-COOH). Attaching proteins such as antibodies or streptavidin (to enable biotinylated linkage) will create highly selective fluorescent probes, which can target specific molecules of interest. The immense brightness of CPNs™ allows detection of molecules with a high degree of sensitivity. Single molecules can be detected using a wide variety of applications, including flow cytometry, immunocytochemistry and histochemistry methods, and ELISA.
The affinity of targeting molecules varies greatly and an initial titration for the CPNs™:targeting molecule ratio must be undertaken. Similarly, final usage dilution of the CPNs™ in the targeting molecule conjugate will need to be determined empirically.