Conjugated Polymer Nanoparticles (CPNs™) can be used in western blot analyses to detect a protein of interest. When linked to key antibodies, the powerful brightness of CPNs™ offers enhanced detection sensitivity, improving the detection limit to clearly and effectively identify the protein, even at low concentrations.
Experimental data shows that CPNs™ can produce a strong and robust signal in western blot analysis, allowing clear identification of the protein or proteins of interest, visible even after storage for extended periods of time. As a result, CPNs™ prove useful tools for sample analysis and protein detection in a range of molecular biology applications.
A. CPN™ western blot result using anti-mouse conjugated CPN™ 510B (green) to detect a mouse-anti-CD44 protein sample. Signal visualised by ChemiDoc (BioRad). B. CPN™ western blot result using anti-mouse conjugated CPN™ 900 (IR-I) to detect a mouse-anti-CD44 protein sample. Signal visualised using an Amersham ImageQuant 800 (Cytiva).